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Dictyostelium discoideum is a well-establish eukaryotic model organism that offers unique advantages for studying fundamental cellular processes, including signal transduction, random and directed cell motility, cytokinesis, endocytosis and vesicle transport and development. Dictyostelium is also increasingly used for the investigation of human disease genes and the crosstalk between host and pathogen. The availability of the genome sequence at a mouse click together with a whole range of supporting information and resources along with a powerful armoury of molecular genetics techniques have considerably enhanced the experimental attractiveness of D. discoideum in recent years.   The second edition of Dictyostelium discoideum Protocols incorporates the most recent developments in a number of fields. The book is divided into four parts. The first part provides an introduction to the amoebozoa and community resources. The second part presents large-scale analysis methods made possible by the completion of the Dictyostelium genome sequence. The third part is dedicated to molecular genetics techniques, cell biological, biochemical and biophysical methods. The chapters in the fourth part describe the use of Dictyostelium as a model system to study vesicle formation, trafficking and infection by bacterial pathogens.   Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Dictyostelium discoideum Protocols, Second Edition seeks to aid scientists in the application of innovating techniques to study a range of fundamental biological processes in this attractive model organism.

Dictyostelium discoideum --- Models, Theoretical --- Publication Formats --- Dictyosteliida --- Biology --- Investigative Techniques --- Biological Science Disciplines --- Publication Characteristics --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Mycetozoa --- Natural Science Disciplines --- Amoebozoa --- Disciplines and Occupations --- Eukaryota --- Organisms --- Laboratory Manuals --- Models, Biological --- Dictyostelium --- Genetic Techniques --- Models, Genetic --- Genetics --- Health & Biological Sciences --- Microbiology & Immunology --- Cytogenetics --- Biologic Sciences --- Biological Science --- Science, Biological --- Sciences, Biological --- Biological Sciences --- Life Sciences --- Biologic Science --- Biological Science Discipline --- Discipline, Biological Science --- Disciplines, Biological Science --- Life Science --- Science Discipline, Biological --- Science Disciplines, Biological --- Science, Biologic --- Science, Life --- Sciences, Biologic --- Sciences, Life --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Dictyostelid Cellular Slime Molds --- Slime Molds, Cellular --- Cellular Slime Mold --- Cellular Slime Molds --- Dictyosteliidas --- Mold, Cellular Slime --- Molds, Cellular Slime --- Slime Mold, Cellular --- Experimental Model --- Experimental Models --- Mathematical Model --- Model, Experimental --- Models (Theoretical) --- Models, Experimental --- Models, Theoretic --- Theoretical Study --- Mathematical Models --- Model (Theoretical) --- Model, Mathematical --- Model, Theoretical --- Models, Mathematical --- Studies, Theoretical --- Study, Theoretical --- Theoretical Model --- Theoretical Models --- Theoretical Studies --- Genetic Models --- Genetic Model --- Model, Genetic --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Dictyostelium discoideums --- Dictyosteliums --- discoideum, Dictyostelium --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Biological Model --- Biological Models --- Model, Biological --- Models, Biologic --- Biologic Model --- Biologic Models --- Model, Biologic --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- methods --- Eumycetozoa --- Eumycetozoas --- Mycetozoas --- Life sciences. --- Microbiology. --- Life Sciences. --- Eukaryotic Microbiology. --- Microbial biology --- Microorganisms --- Biosciences --- Science

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This volume contains a selection of chapters based on papers presented at the Fourth Seattle Symposium on Biostatistics: Clinical Trials. These biostatistical symposiums, which unite leading researchers every five years, represent important developments in field. The Fourth Seattle Symposium was held in 2010 to celebrate the 40th anniversary of the University of Washington School of Public Health and Community Medicine. The Symposium featured keynote lectures by Robert O’Neill, Ross Prentice and Robert Temple, as well as invited talks by Jesse Berlin, Christy Chuang-Stein, David DeMets, Bill DuMouchel, Susan Ellenberg, Thomas Fleming, Laurence Freedman, Margaret Pepe, Steve Self, Richard Simon, Bruce Weir, John Whittaker and Janet Wittes.  Invited panelists included Jesse Berlin, Bruce Binkowitz, Christy Chuang-Stein, Bill DuMouchel, Susan Ellenberg, Thomas Fleming, Henry Fuchs, Dominic Labriola, Robert O’Neill, Robert Temple and Janet Wittes. The thoroughly peer-reviewed papers and material from short courses that are showcased in this volume represent the theme of the symposium, clinical trials. These papers encompass recent methodological advances on several important topics, summaries of the state of the art of methodology in key areas of clinical trials, as well as innovative applications of the existing theory and methods. This volume will be a valuable reference for researchers and practitioners in the field of clinical trials.

Biometry -- Data processing. --- Biometry. --- Biostatistics. --- Medical statistics -- Data processing. --- Medical statistics. --- Biometry --- Statistics as Topic --- Epidemiologic Methods --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Biostatistics --- Mathematics --- Biology --- Physical Sciences & Mathematics --- Health & Biological Sciences --- Mathematical Statistics --- Biology - General --- Biological Statistics --- Biological Statistic --- Statistic, Biological --- Statistics, Biological --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Epidemiologic Method --- Epidemiological Methods --- Methods, Epidemiologic --- Epidemiological Method --- Method, Epidemiologic --- Method, Epidemiological --- Methods, Epidemiological --- Epidemiology --- Area Analysis --- Correlation Studies --- Correlation Study --- Correlation of Data --- Data Analysis --- Estimation Technics --- Estimation Techniques --- Indirect Estimation Technics --- Indirect Estimation Techniques --- Multiple Classification Analysis --- Service Statistics --- Statistical Study --- Statistics, Service --- Tables and Charts as Topic --- Analyses, Area --- Analyses, Data --- Analyses, Multiple Classification --- Analysis, Area --- Analysis, Data --- Analysis, Multiple Classification --- Area Analyses --- Classification Analyses, Multiple --- Classification Analysis, Multiple --- Data Analyses --- Data Correlation --- Data Correlations --- Estimation Technic --- Estimation Technic, Indirect --- Estimation Technics, Indirect --- Estimation Technique --- Estimation Technique, Indirect --- Estimation Techniques, Indirect --- Indirect Estimation Technic --- Indirect Estimation Technique --- Multiple Classification Analyses --- Statistical Studies --- Studies, Correlation --- Studies, Statistical --- Study, Correlation --- Study, Statistical --- Technic, Estimation --- Technic, Indirect Estimation --- Technics, Estimation --- Technics, Indirect Estimation --- Technique, Estimation --- Technique, Indirect Estimation --- Techniques, Estimation --- Techniques, Indirect Estimation --- methods --- Statistics. --- Statistics, general. --- Statistics for Life Sciences, Medicine, Health Sciences. --- Statistical analysis --- Statistical data --- Statistical methods --- Statistical science --- Econometrics --- Statistics .

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Victor P. Bulgakov, Yuri N. Shkryl, Galina N. Veremeichik, Tatiana Y. Gorpenchenko and Yuliya V. Vereshchagina: Recent Advances in the Understanding of Agrobacterium rhizogenes-Derived Genes and Their Effects on Stress Resistance and Plant Metabolism. Le Zhao, Guy W. Sander and Jacqueline V. Shanks: Perspectives of the Metabolic Engineering of Terpenoid Indole Alkaloids in Catharanthus roseus Hairy Roots. Jian Wen Wang and Jian Yong Wu: Effective Elicitors and Process Strategies for Enhancement of Secondary Metabolite Production in Hairy Root Cultures. Amanda R. Stiles and Chun-Zhao Liu: Hairy Root Culture: Bioreactor Design and Process Intensification. Marina Skarjinskaia, Karen Ruby, Adriana Araujo, Karina Taylor, Vengadesan Gopalasamy-Raju, Konstantin Musiychuk, Jessica A. Chichester, Gene A. Palmer, Patricia de la Rosa, Vadim Mett, Natalia Ugulava, Stephen J. Streatfield and Vidadi Yusibov: Hairy Roots as a Vaccine Production and Delivery System. Zahwa Al-Shalabi and Pauline M. Doran: Metal Uptake and Nanoparticle Synthesis in Hairy Root Cultures.

Roots (Botany) --- Root hairs --- Investigative Techniques --- Biology --- Plant Structures --- Physiological Processes --- Metabolic Phenomena --- Equipment and Supplies --- Biological Science Disciplines --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Phenomena and Processes --- Physiological Phenomena --- Anatomy --- Natural Science Disciplines --- Disciplines and Occupations --- Plant Roots --- Metabolism --- Bioreactors --- Growth and Development --- Methods --- Genetics --- Health & Biological Sciences --- Pharmacy, Therapeutics, & Pharmacology --- Microbiology & Immunology --- Research --- Methodological Studies --- Methodological Study --- Procedures --- Studies, Methodological --- Study, Methodological --- Method --- Procedure --- Development and Growth --- Fermentors --- Bioreactor --- Fermentor --- Metabolic Phenomenon --- Metabolic Process --- Metabolism Concepts --- Metabolism Phenomena --- Process, Metabolic --- Processes, Metabolic --- Anabolism --- Catabolism --- Metabolic Concepts --- Metabolic Processes --- Concept, Metabolic --- Concept, Metabolism --- Concepts, Metabolic --- Concepts, Metabolism --- Metabolic Concept --- Metabolism Concept --- Phenomena, Metabolic --- Phenomena, Metabolism --- Phenomenon, Metabolic --- Plant Bulbs --- Plant Root --- Bulb, Plant --- Bulbs, Plant --- Plant Bulb --- Root, Plant --- Roots, Plant --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Anatomies --- Physiological Concepts --- Physiological Phenomenon --- Physiological Process --- Concept, Physiological --- Concepts, Physiological --- Phenomena, Physiological --- Phenomenas, Physiological --- Phenomenon, Physiological --- Physiological Concept --- Process, Physiological --- Processes, Physiological --- Biologic Sciences --- Biological Science --- Science, Biological --- Sciences, Biological --- Biological Sciences --- Life Sciences --- Biologic Science --- Biological Science Discipline --- Discipline, Biological Science --- Disciplines, Biological Science --- Life Science --- Science Discipline, Biological --- Science Disciplines, Biological --- Science, Biologic --- Science, Life --- Sciences, Biologic --- Sciences, Life --- Device, Medical --- Devices, Medical --- Equipment --- Inventories --- Medical Device --- Supplies --- Apparatus and Instruments --- Devices --- Medical Devices --- Device --- Instruments and Apparatus --- Inventory --- Supplies and Equipment --- Plant Components --- Component, Plant --- Components, Plant --- Plant Component --- Plant Structure --- Structure, Plant --- Structures, Plant --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Medicine. --- Vaccines. --- Biotechnology. --- Plant genetics. --- Biomedicine. --- Vaccine. --- Plant Genetics & Genomics. --- Root hairs. --- Research. --- Plant Genetics and Genomics. --- Plants --- Chemical engineering --- Genetic engineering --- Biologicals

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The field of regenerative medicine has developed rapidly over the past 20 years with the advent of molecular and cellular techniques. This textbook,  Regenerative Medicine: From Protocol to Patient, aims to explain the scientific knowledge and emerging technology as well as the clinical application in different organ systems and diseases. International leading experts from four continents describe the latest scientific and clinical knowledge of the field of regenerative medicine. The process of translating science of laboratory protocols into therapies is explained in sections on regulatory, ethical and industrial issues.   This textbook is organized into five parts: (I) Biology of Tissue Regeneration, (II) Stem Cell Science and Technology, (III) Tissue Engineering, Biomaterials and Nanotechnology, (IV) Regenerative Therapies and (V) Regulation and Ethics.   The textbook aims to give the student, the researcher, the health care professional, the physician and the patient a complete survey on the current scientific basis, therapeutic protocols, clinical translation and practiced therapies in regenerative medicine.

Cellular therapy. --- Medical -- Biotechnology. --- Regenerative medicine. --- Medicine --- Biomedical Research --- Investigative Techniques --- Biological Processes --- Cells --- Research --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Biological Phenomena --- Anatomy --- Health Occupations --- Disciplines and Occupations --- Science --- Phenomena and Processes --- Natural Science Disciplines --- Regeneration --- Regenerative Medicine --- Stem Cell Research --- Stem Cells --- Colony-Forming Unit --- Colony-Forming Units --- Mother Cells --- Progenitor Cells --- Cell, Mother --- Cell, Progenitor --- Cell, Stem --- Cells, Mother --- Cells, Progenitor --- Cells, Stem --- Colony Forming Unit --- Colony Forming Units --- Mother Cell --- Progenitor Cell --- Stem Cell --- Adult Stem Cell Research --- Embryonic Stem Cell Research --- Research, Stem Cell --- Researchs, Stem Cell --- Stem Cell Researchs --- Medicine, Regenerative --- Medicines, Regenerative --- Regenerative Medicines --- Regenerations --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Sciences --- Health Professions --- Health Occupation --- Health Profession --- Occupation, Health --- Occupations, Health --- Profession, Health --- Professions, Health --- Anatomies --- Biologic Phenomena --- Biological Phenomenon --- Biological Process --- Phenomena, Biological --- Phenomena, Biologic --- Phenomenon, Biological --- Process, Biological --- Processes, Biological --- Laboratory Research --- Research Activities --- Research and Development --- Research Priorities --- Activities, Research --- Activity, Research --- Development and Research --- Priorities, Research --- Priority, Research --- Research Activity --- Research Priority --- Research, Laboratory --- Cell --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Experimental Medicine --- Investigational Medicine --- Investigative Medicine --- Research, Biomedical --- Research, Medical --- Medical Research --- Medicine, Experimental --- Medicine, Investigational --- Medicine, Investigative --- Medical Specialities --- Medical Specialties --- Medical Specialty --- Specialities, Medical --- Specialties, Medical --- Specialty, Medical --- Medical Speciality --- Speciality, Medical --- Medicine. --- Surgery. --- Stem cells. --- Nanotechnology. --- Biomedicine. --- Biomedicine general. --- Stem Cells. --- Molecular technology --- Nanoscale technology --- High technology --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Surgery, Primitive --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Biomedicine, general. --- Health Workforce

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The new edition of this introductory-level textbook continues to offer a concise and approachable bridge between student lecture notes and more detailed clinical reference works. All aspects of equine medicine, surgery and reproduction are covered in a single, convenient volume. The second edition has been subject to an extensive revision, with each chapter updated and new chapters added to cover wound management, critical care, anaesthesia and sedation, and diagnostic imaging. While offering key information in an easily and quickly digested format for clinical veterinary students and practis

Horses -- Diseases. --- Horses -- Reproduction. --- Horses -- Surgery. --- Veterinary medicine. --- Veterinary surgery. --- Equidae --- Reproductive Physiological Processes --- Investigative Techniques --- Veterinary Medicine --- Animal Diseases --- Diseases --- Perissodactyla --- Reproductive Physiological Phenomena --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Health Occupations --- Disciplines and Occupations --- Reproductive and Urinary Physiological Phenomena --- Mammals --- Phenomena and Processes --- Vertebrates --- Chordata --- Animals --- Eukaryota --- Organisms --- Horse Diseases --- Methods --- Horses --- Reproduction --- Surgery, Veterinary --- Equus caballus --- Equus przewalskii --- Horse, Domestic --- Domestic Horse --- Domestic Horses --- Horse --- Horses, Domestic --- Methodological Studies --- Methodological Study --- Procedures --- Studies, Methodological --- Study, Methodological --- Method --- Procedure --- Equine Diseases --- Disease, Equine --- Disease, Horse --- Diseases, Equine --- Diseases, Horse --- Equine Disease --- Horse Disease --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Animalia --- Animal --- Metazoa --- Chordates --- Chordate --- Vertebrate --- Mammalia --- Mammal --- Physiology, Reproductive and Urinary --- Reproductive and Urinary Physiological Phenomenon --- Reproductive and Urinary Physiology Concepts --- Reproductive and Urinary Physiology Phenomenon --- Reproductive and Urinary Tract Physiological Concepts --- Reproductive and Urinary Tract Physiological Phenomena --- Reproductive and Urinary Tract Physiological Phenomenon --- Reproductive and Urinary Tract Physiology --- Reproductive and Urinary Physiology --- Reproductive and Urinary Physiology Phenomena --- Health Professions --- Health Occupation --- Health Profession --- Profession, Health --- Professions, Health --- Occupations --- Reproductive Physiologic Concepts --- Reproductive Physiological Phenomenon --- Reproductive Physiological Process --- Reproductive Physiological Concepts --- Concept, Reproductive Physiologic --- Concept, Reproductive Physiological --- Concepts, Reproductive Physiologic --- Concepts, Reproductive Physiological --- Phenomena, Reproductive Physiological --- Phenomenon, Reproductive Physiological --- Physiologic Concept, Reproductive --- Physiologic Concepts, Reproductive --- Physiological Concept, Reproductive --- Physiological Process, Reproductive --- Physiological Processes, Reproductive --- Process, Reproductive Physiological --- Processes, Reproductive Physiological --- Reproductive Physiologic Concept --- Reproductive Physiological Concept --- Perissodactylas --- Diseases, Animal --- Disease --- Medicine, Veterinary --- Animal Husbandry --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Ass --- Equus asinus --- Asses --- Donkeys --- Mules --- Zebras --- Donkey --- Mule --- Zebra --- Veterinary Surgery --- Surgeries, Veterinary --- Veterinary Surgeries --- Human Reproductive Index --- Human Reproductive Indexes --- Reproductive Period --- Human Reproductive Indices --- Index, Human Reproductive --- Indexes, Human Reproductive --- Indices, Human Reproductive --- Period, Reproductive --- Periods, Reproductive --- Reproductive Index, Human --- Reproductive Indices, Human --- Reproductive Periods --- veterinary --- Horse Diseases. --- Reproduction. --- Horses. --- methods. --- Techniques --- Technique --- methods